Table 2 DNA-based methods of meat authentification in 2023
Method | Target | Reference |
|---|---|---|
DNA-based methods (systematic review) | Game and less common meat species | Adenuga et al., 2023 |
PCR | Broadhead catfish (Clarias macrocephalus), African catfish (Clarias gariepinus), and black eel-tail catfish (Plotosus canius) | Benjakul et al., 2023 |
PCR | Frog meat (specificity against common halal meat sources such as beef, chicken, shrimp, squid, and mackerel) | Haryono et al., 2023 |
Microarray chip PCR-directed microfluidic lateral flow strip (LFS) device | Beef adulterated with chicken, duck, and pork | Wang et al., 2023a |
Real-time PCR | Different meat species in meat products marketed as beef | Özlü et al., 2023 |
Duplex PCR | Cattle and pork | Barido et al., 2023 |
Triplex real-time PCR | Meats and antlers from sika deer (Cervus nippon) and red deer (Cervus elaphus) | Liu et al., 2023b |
Multiplex-PCR | Deer, cow, sheep, pig and horse | Wang et al., 2023f |
Multiplex-PCR | Columba livia, Corvus moneduloides, Gallus gallus, Coturnix japonica, Phasianus colchicus, Struthio camelus, and Meleagris gallopavo meats | Rajaei & Doosti, 2023 |
Multiplex-PCR | Cattle and buffalo meat | Karabasanavar et al., 2023 |
Multiplex-PCR | Bovine, chicken, porcine, dog, and rat ingredients in beef products | Wibowo et al., 2023 |
Multiplex-PCR using a polydimethylsiloxane microfluidic device | Beef, chicken, pork, and duck | Yu et al., 2023a |
Direct quantitative real-time PCR (qPCR) | Pork in processed meat products | Kusnadi et al., 2023 |
qPCR | Rat in meat | Ji et al., 2023 |
TaqMan multiplex qPCR (multiplex real-time polymerase chain reaction (mqPCR)) | Meat products (review) | Hossain et al., 2023 |
High-resolution melt analysis (HRMA) | Commonly used meat species | Jafar et al., 2023 |
Droplet digital PCR (ddPCR) | Pork in cattle | Milli et al., 2023 |
ddPCR | Beef (Bos taurus) and lamb (Ovis aries) | He et al., 2023b |
ddPCR | Buffalo substitution in 'Haleem' | Kumar et al., 2023a |
qPCR and ddPCR | Dominant meat species (pork, chicken and beef) in samples of 2- and 3-component mixtures and meat products | Nesvadbova et al., 2023 |
DNA-metabarcoding | Different taxa | Denay et al., 2023 |
Real-time fluorescent loop-mediated isothermal amplification (LAMP)-microfluidic assay | Pork, beef, sheep and duck in foodstuffs | Zhang et al., 2023a |
Real-time enzymatic recombinase amplification (real-time ERA) | Horse-, donkey- and pig-related meat products | Zhou et al., 2023 |
Integrating microneedle DNA extraction to hand-held microfluidic colorimetric (visual) LAMP system | Goat meat, sheep meat, pork meat, chicken meat, donkey meat, and duck meat | Xiao et al., 2023 |
Recombinase polymerase amplification (RPA) combined with CRISPR/Cas12a technology | Chicken, duck, beef, pork and lamb | Liu et al., 2023f |
RPA combined with CRISPR/Cas12a | Beef, mutton, pork, chicken, and duck | Ding et al., 2023 |
Isothermal polymerase spiral reaction (PSR) | Buffalo (Bubalus bubalus) | Jawla & Chatli, 2024 |
PSR | Goat (Capra hircus) | Shree et al., 2023 |
PSR | Pig | Danawadkar et al., 2023 |
Dual-recombinase-aided amplification (dual-RAA) technology and visual multiplex lateral flow strips (MLFSs) | Duck- and bovine-derived, porcine- and bovine-derived, duck- and ovine-derived, and porcine- and ovine-derived meat | Cao & Song, 2023 |